Closed-loop control of vortex shedding by means of Lorentz force

When an incompressible fluid flows past a circular cylinder, vortex shedding occurs as soon as the Reynolds number exceeds about 40. Vortex shedding is usually undesirable, as it generates a significant increase in drag, as well an oscillating lift force on the cylinder leading to cross-stream structural vibrations. Flow control to either delay the appearance of vortex shedding or fully suppress it has attracted much attention during the last decade. The focus of this dissertation is to control vortex shedding from a circular cylinder by applying an external electromagnetic field. As in previous works, the latter is generated by electrodes and magnets alternatively arranged on the cylinder surface. In a weakly conducting fluid such as seawater, this has the effect of creating a Lorentz force tangential to the surface of the cylinder and oriented in the flow direction. A novel analytical expression of the Lorentz force is derived by integrating the Maxwell equations and using series expansions. This expression is then used for the control of vortex shedding in numerical simulations. Specifically, a closed-loop control algorithm is derived utilizing a single point sensor on the cylinder surface and the bilinear searching method in order to determine the appropriate magnitude of the Lorentz force at every time. Numerical simulations based on a two-dimensional Navier-Stokes solver show that vortex shedding is indeed suppressed at the Reynolds number values Re = 100 and 200

Effects of the New Cooperative Medical Scheme on village doctor’s prescribing behaviour in Shandong Province

Objective: To assess the effects of China’s new community health insurance, the New Cooperative Medical Scheme (NCMS), on village doctors’ prescribing behaviour. NCMS began in 2003. Method, In 2005 we conducted a quasi-experimental case-control study in Shandong Province, and collected information from 2,271 patient visits in 30 village health stations. Results, NCMS has adversely influenced prescribing behaviour of village doctors. Average number of drugs prescribed, percentage of prescriptions containing antibiotics, number of antibiotics per prescription, percentage of patients given injections, and average per prescription cost were consistently higher in NCMS village health stations than non-NCMS. Within NCMS villages, prescribing behaviour towards insured patients was significantly different to the uninsured. Conclusion, Over-prescribing is common in villages with and without health insurance, with grave concerns for service quality and drug-use safety. Policy implications are NCMS should be redesigned to exert more influence on health providers, with incentives for cost containment and service quality. Stricter regulatory environment for prescriptions is necessary to counter irrational drug-use and ensure people’s access to effective care at reasonable cost.

Failure Mode and Effect Analysis of Power Transformer Based on Cloud Model of Weight

As the key component of a power system, the power transformer directly impacts the reliability and safety of the system. Failure mode and effects analysis (FMEA) is a methodology used to analyze potential failure modes within a system and has been used extensively to examine the power transformer’s performance in various potential failure scenarios. However, the FMEA method has several flaws; for example, the non-differential analysis of evaluation index and the impossibility of evaluating the actual risk among risk priority number (RPN) values that on the surface are equal. The cloud model of weight incorporates the relative importance of index. This paper proposes applying FMEA based on the cloud model of weight to assess a power transformer for risk, and shows that this method can effectively overcome the defects of traditional FMEA assessment methods

Antibodies against outer-capsid proteins of grass carp reovirus expressed in E. coli are capable of neutralizing viral infectivity

<p>Abstract</p> <p>Background</p> <p>Grass carp reovirus (GCRV), which causes severe infectious outbreaks of hemorrhagic disease in aquatic animals, is a highly pathogenic agent in the <it>Aquareovirus </it>genus of family <it>Reoviridae</it>. The outer capsid shell of GCRV, composed of the VP5-VP7 protein complex, is believed to be involved in cell entry. The objective of this study was to produce a major neutralization antibody for mitigating GCRV infection.</p> <p>Results</p> <p>Recombinant plasmids of GCRV outer capsid proteins VP5 and VP7 were constructed and expressed in prokaryotic cells in our previous work. In this study, we prepared GCRV Antibody (Ab), VP5Ab and VP7Ab generated from purified native GCRV, recombinant VP5 and VP7 respectively. Immunoblotting analysis showed that the prepared antibodies were specific to its antigens. In addition, combined plaque and cytopathic effect (CPE)-based TCID₅₀(50% tissue culture infective dose) assays showed that both VP5Ab and VP7Ab were capable of neutralizing viral infectivity. Particularly, the neutralizing activity of VP7Ab was 3 times higher than that of VP5Ab, suggesting that VP7 might be a dominating epitope. Moreover, the combination of VP5Ab and VP7Ab appeared to enhance GCRV neutralizing capacity.</p> <p>Conclusions</p> <p>The results presented in this study indicated that VP7 protein was the major epitope of GCRV. Furthermore, VP5Ab and VP7Ab in combination presented an enhanced capacity to neutralize the GCRV particle, suggesting that the VP5 and VP7 proteins may cooperate with each other during virus cell entry. The data can be used not only to further define the surface epitope domain of GCRV but may also be applicable in the designing of vaccines.</p

Functional investigation of grass carp reovirus nonstructural protein NS80

<p>Abstract</p> <p>Background</p> <p>Grass Carp Reovirus (GCRV), a highly virulent agent of aquatic animals, has an eleven segmented dsRNA genome encased in a multilayered capsid shell, which encodes twelve proteins including seven structural proteins (VP1-VP7), and five nonstructural proteins (NS80, NS38, NS31, NS26, and NS16). It has been suggested that the protein NS80 plays an important role in the viral replication cycle that is similar to that of its homologous protein μNS in the genus of <it>Orthoreovirus</it>.</p> <p>Results</p> <p>As a step to understanding the basis of the part played by NS80 in GCRV replication and particle assembly, we used the yeast two-hybrid (Y2H) system to identify NS80 interactions with proteins NS38, VP4, and VP6 as well as NS80 and NS38 self-interactions, while no interactions appeared in the four protein pairs NS38-VP4, NS38-VP6, VP4-VP4, and VP4-VP6. Bioinformatic analyses of NS80 with its corresponding proteins were performed with all currently available homologous protein sequences in ARVs (avian reoviruses) and MRVs (mammalian reoviruses) to predict further potential functional domains of NS80 that are related to VFLS (viral factory-like structures) formation and other roles in viral replication. Two conserved regions spanning from aa (amino acid) residues of 388 to 433, and 562 to 580 were discovered in this study. The second conserved region with corresponding conserved residues Tyr565, His569, Cys571, Asn573, and Glu576 located between the two coiled-coils regions (aa ~513-550 and aa ~615-690) in carboxyl-proximal terminus were supposed to be essential to form VFLS, so that aa residues ranging from 513 to 742 of NS80 was inferred to be the smallest region that is necessary for forming VFLS. The function of the first conserved region including Ala395, Gly419, Asp421, Pro422, Leu438, and Leu443 residues is unclear, but one-third of the amino-terminal region might be species specific, dominating interactions with other viral components.</p> <p>Conclusions</p> <p>Our results in this study together with those from previous investigations indicate the protein NS80 might play a central role in VFLS formation and viral components recruitment in GCRV particle assembly, similar to the μNS protein in ARVs and MRVs.</p

Mining Sequential Relations from Multidimensional Data Sequence for Prediction

By analyzing historical data sequences and identifying relations between the occurring of data items and certain types of business events we have opportunities to gain insights into future status and thereby take action proactively. This paper proposes a new approach to cope with the problem of prediction on data sequence characterized by multiple dimensions. The proposed relation mining approach improves the existing sequential pattern mining algorithm by considering multidimensional data sequences and incorporating time constraints. We demonstrate that multidimensional relations extracted by our approach are an enhancement of single dimensional relations by showing significantly stronger prediction capability, despite of the substantial work done in the latter area. In addition, matching algorithm based on the obtained relations is proposed to make prediction. The effectiveness of the proposed methods is validated by experiments conducted on a mobile user context dataset

Mga Modulates Bmpr1a Activity by Antagonizing Bs69 in Zebrafish

MAX giant associated protein (MGA) is a dual transcriptional factor containing both T-box and bHLHzip DNA binding domains. In vitro studies have shown that MGA functions as a transcriptional repressor or activator to regulate transcription of promotors containing either E-box or T-box binding sites. BS69 (ZMYND11), a multidomain-containing (i.e., PHD, BROMO, PWWP, and MYND) protein, has been shown to selectively recognizes histone variant H3.3 lysine 36 trimethylation (H3.3K36me3), modulates RNA Polymerase II elongation, and functions as RNA splicing regulator. Mutations in MGA or BS69 have been linked to multiple cancers or neural developmental disorders. Here, by TALEN and CRISPR/Cas9-mediated loss of gene function assays, we show that zebrafish Mga and Bs69 are required to maintain proper Bmp signaling during early embryogenesis. We found that Mga protein localized in the cytoplasm modulates Bmpr1a activity by physical association with Zmynd11/Bs69. The Mynd domain of Bs69 specifically binds the kinase domain of Bmpr1a and interferes with its phosphorylation and activation of Smad1/5/8. Mga acts to antagonize Bs69 and facilitate the Bmp signaling pathway by disrupting the Bs69-Bmpr1a association. Functionally, Bmp signaling under control of Mga and Bs69 is required for properly specifying the ventral tailfin cell fate.</p

PPIRank - an advanced method for ranking protein-protein interations in TAP/MS data

Background: Tandem affinity purification coupled with mass-spectrometry (TAP/MS) analysis is a popular method for the identification of novel endogenous protein-protein interactions (PPIs) in large-scale. Computational analysis of TAP/MS data is a critical step, particularly for high-throughput datasets, yet it remains challenging due to the noisy nature of TAP/MS data. Results: We investigated several major TAP/MS data analysis methods for identifying PPIs, and developed an advanced method, which incorporates an improved statistical method to filter out false positives from the negative controls. Our method is named PPIRank that stands for PPI ranking in TAP/MS data. We compared PPIRank with several other existing methods in analyzing two pathway-specific TAP/MS PPI datasets from Drosophila. Conclusion: Experimental results show that PPIRank is more capable than other approaches in terms of identifying known interactions collected in the BioGRID PPI database. Specifically, PPIRank is able to capture more true interactions and simultaneously less false positives in both Insulin and Hippo pathways of Drosophila Melanogaster

Alleviation of cadmium toxicity in cucumber (Cucumis sativus) seedlings by the application of selenium

In the present study, the role of selenium in cadmium toxicity was investigated in cucumber seedlings by hydroponic experiments. The application of Se for cucumber exposed to Cd significantly reduced Cd accumulation in all tissues, elevated Cd-depressed chlorophyll content, and improved photosynthetic performance. External Se significantly reduced ·OH, H2O2 and malondialdehyde content. Exogenous Se balanced Cd-depressed elements (e.g., Se enhanced Cd-induced decreases in root Zn, leaf/stem/root Mn concentrations) and carbohydrate contents. External Se also significantly decreased the Cd-induced increases in Na+K+-, Ca2+Mg2+- and total ATPase activities, which recovered almost to control level. Results indicate that application of Se can alleviate Cd toxicity in cucumber seedlings by reducing Cd uptake and reactive oxygen species (ROS) accumulation, moreover protecting photosynthetic machinery from damaging, balancing elements and carbohydrate contents, and improving ATPase activities in cucumber